[3dem] Electron cryo-tomography on polymers

[T.Sharp at lumc.nl]

Dear all,

Thanks for all the helpful responses, although I perhaps should have been clearer in my original email!
I’m used to imaging biological samples (proteins, liposomes, etc) but will be looking at non-biological organic polymers (specifically a bolaamphiphile).

Unlike for biological sciences where typically each parameter is reported, the papers I’ve found where cryoET has been used to image organic polymers do not state the total dose used, and a few papers mention sparse-sampling tilt angles of 5 degrees or more.
In my limited experience of imaging these types of sample they appear to be more resistant to damage than proteins, so I’m really looking for any advice from people who have imaged organic polymers before.
At the end of the day, they are still composed of C,H,N and O in water, so I’ll start with my default collection scheme and see how things go!

Thanks again,

Thom

Dr. Thomas H. Sharp

• 0031-(0)-7152-69499
• t.sharp at lumc.nl
https://electronmicroscopy.lumc.nl/
________________________________
From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of T.Sharp at lumc.nl [T.Sharp at lumc.nl]
Sent: 02 December 2015 15:54
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Electron cryo-tomography on polymers

Dear all,

Has anyone performed electron cryo-tomography on polymers before? I’m curious about what dose those sorts of samples can withstand, and whether a different tilt scheme is beneficial. There’s some info in the paper below but it’s simulated data, so it would be great to hear from anyone with practical experience.

On Resolution in Electron Tomography of Beam Sensitive Materials. D. Chen, H. Friedrich and G. de With. J. Phys. Chem. C, 2014, 118 (2), pp 1248–1257. DOI: 10.1021/jp409185u

Thanks in advance,

Thom

Dr. Thomas H. Sharp

Leiden University Medical Center
Section Electron Microscopy
Leiden, NL

• 0031-(0)-7152-69499
• t.sharp at lumc.nl<mailto:t.sharp at lumc.nl>
https://electronmicroscopy.lumc.nl/

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