[3dem] Relion and Random Conical Tilt Reconstructions

[Carlos Oscar S. Sorzano]

Dear Fabien,

in principle, this can be done with Scipion 
(http://scipion.cnb.csic.es/docs/bin/view/TWiki/WebHome). Scipion is not 
officially released yet (although a beta version will be soon). Scipion 
converts the 2D alignment parameters produced by Relion into alignment 
parameters that Xmipp RCT can use. Once it is public, we will be happy 
to help you installing and using the framework.

Kind regards, Carlos Oscar

On 04/09/2015 09:41 PM, Fabien Alexandre Bergeret, Dr wrote:
> Dear 3dem,
>
> I am currently doing random conical tilt reconstructions with 
> Xmipp3.1. So far, 2D classifications with CL2D gave me the best 
> results. I've also made 2D classifications of my untilted particles 
> with Relion and it gave me very good classifications (nice classes and 
> nice particles inside the classes).
> I would like to use the 2D classifications from Relion for doing the 
> RCT reconstructions with Xmipp. I don't know if it is possible and if 
> yes how? My concerns are for example about the difference of particles 
> numbering between Xmipp and Relion and the differences between the 
> metafile formats.
> Any help and suggestions are welcome ;)
> Thanks in advance.
> Best,
> Fabien
>
>
>
>
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-- 
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano                  e-mail:   coss at cnb.csic.es
Biocomputing unit                             http://biocomp.cnb.csic.es
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco)     Tlf: 34-91-585 4510
28049 MADRID (SPAIN)                          Fax: 34-91-585 4506
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