[3dem] Vitrobot: Humidifier error "empty"

Wim Hagen hagen at embl.de
Wed Sep 24 13:54:42 PDT 2014

Hi Rogelio,

On older models (at least Mark2)  this is the original humidifier: http://www.reptilica.de/Hobby-Hygro-Plus-terrarium-fogger.phtml

Judging from the fancy error message I assume you have a Mark4, no idea what humidifier is used for those but since the machine complains about level, maybe it is using a simpel floater which might be stuck after cleaning, I would try a gentle "technical-hammer-hit”, nothing to loose….

A sponge in a small cup of water placed in the chamber left of the blot pads wil give 85% to 90% RHM at room temperature on our Mark2. Here’s an example of one of our manual blotting devices which also uses the sponge-in-cup, this one gets to 85%:


Wim Hagen
EMBL Heidelberg

On Sep 24, 2014, at 8:04 PM, Rogelio A. Hernandez-Lopez <rhernand at fas.harvard.edu> wrote:

> Hi All,
> We are experiencing a problem getting the humidifier to work after cleaning the water cylinder 
> and expect it might have something to do with the connection between the  water cylinder 
> and the system. As soon as we try to increase the humidity in the chamber the systems trows a message saying 
> "empty" (in the humidity panel). We have already tried emptying and refilling it again and removing any air bubble in the line but that has not fixed the problem.
> Has anyone else had this problem and if so how did you solve it? 
> Thanks.
> Rogelio
> -- 
> Rogelio A. Hernandez Lopez
> Leschziner Lab 
> Harvard University
> 305.G NWB
> 52 Oxford Street, Room 305
> Cambridge, MA 02138
> rhernand at fas.harvard.edu
> (617)-872-5399
> http://labs.mcb.harvard.edu/leschziner/rogelio/index.html
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> 3dem at ncmir.ucsd.edu
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