[3dem] Vitrobot: Humidifier error "empty"

Bob Grassucci rg2502 at columbia.edu
Wed Sep 24 11:49:47 PDT 2014

Hi Rogelio,
We had this problem but it turned out that the fan in the back of the 
chamber which disperses the humidity was not running and therefore the 
water just sat on top of the entrance and would eventually leak out.
Possibility 2  is that you are not getting any from the outer to inner 
vessel because it is air locked as I was told when I faced this 
problem.   Do not fill the cylinder completely use about 40 ml rather 
than the full 50 suggested in the manual.
Possibility 3 as John R. said could be the sonicator not working but can 
be checked by observing the water surface with the cylinder detached 
(should be vibrating)  Don't get splashed. Either the cyllinder is not 
working or the controller board is shot then you need and alternative 
source of water vapor.
Possibility 4 is that the sensor is faulty and the humidity is at the 
correct level but not registering properly.  Use another sensor to check 
the real humidity level.

Good luck.

On 9/24/2014 2:04 PM, Rogelio A. Hernandez-Lopez wrote:
> Hi All,
> We are experiencing a problem getting the humidifier to work after cleaning the water cylinder
> and expect it might have something to do with the connection between the  water cylinder
> and the system. As soon as we try to increase the humidity in the chamber the systems trows a message saying
> "empty" (in the humidity panel). We have already tried emptying and refilling it again and removing any air bubble in the line but that has not fixed the problem.
> Has anyone else had this problem and if so how did you solve it?
> Thanks.
> Rogelio
> -- 
> Rogelio A. Hernandez Lopez
> Leschziner Lab
> Harvard University
> 305.G NWB
> 52 Oxford Street, Room 305
> Cambridge, MA 02138
> rhernand at fas.harvard.edu <mailto:rhernand at fas.harvard.edu>
> (617)-872-5399
> http://labs.mcb.harvard.edu/leschziner/rogelio/index.html
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