[3dem] Biological TEM Standards

[David Belnap]

This may not be clear from David's message, but you can buy already prepared TEM grids of negatively stained catalase from at least two sources: Ted Pella and Electron Microscopy Sciences.

David Belnap


On Oct 15, 2014, at 7:34 AM, David Gene Morgan wrote:

> Hi,
> 
> 	Someone may have already mentioned this, but catalase is another great biological TEM standard.  It's commercially available, can be recrystallized into TEM-thin platelets and is a great teaching tool for electron diffraction, low dose, cryoTEM, etc.
> 
> On 10/14/2014 05:10 PM, Jonathan Remis wrote:
>> Hello everyone,
>> 
>> We are looking for some standard biological samples to image under negative stained or cryo conditions.  These standards would be used show new TEM users what sort of data they can expect with correctly prepared and handled samples.  I suppose the only requirements for this standard would be MW >250kd, that it stains well with Uranyl Acetate or Uranyl Formate and that it be fairly homogeneous.
>> 
>> Ideally we would like to purchase a standard instead of purify it but I am not having much luck finding what I thought would have been common (GroEL, TMV) TEM standards for sale.  If necessary we will purify our own but I thought maybe someone on this listserv may have more information on how to obtain such a standard. Thank you for your time and help.
>> 
>> Best wishes,
>> Jonathan Remis
>> ________________________________________
>> 
>> JONATHAN REMIS
>> 
>> CryoEM Facilities Manager
>> 
>> Northwestern University
>> Department of Molecular Biosciences
>> 
>> mailing address:
>> 2205 Tech Drive
>> Hogan 2-100
>> Evanston, IL 60208-3500
>> 
>> OFFICE +1-847-467-5258
>> CELL   +1-847-387-2053
>> EMAIL  jonathan.remis at northwestern.edu
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
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> 
> 
> -- 
>                 David Gene Morgan
>             Electron Microscopy Center
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