[3dem] Biological TEM Standards

[bestdz at gmail.com]

Hi Jonathan,

You can buy keyhole limpet hemocyanin, aka KLH, from Sigma Aldrich for less than $100. http://www.sigmaaldrich.com/catalog/product/sigma/h7017?lang=en&region=US

It comes as powder. You just add reconstitution solution and sit overnight, then you will get pretty uniform assemblies around 20nm in size. We’ve been using this sample for years for undergraduate lab courses.  


Best regards,



Xinghong Dai
PhD Candidate
Zhou Lab, MIMG/CNSI, UCLA
+1 310 447 4330





From: Jonathan Remis
Sent: ‎Tuesday‎, ‎October‎ ‎14‎, ‎2014 ‎2‎:‎10‎ ‎PM
To: 3dem at ncmir.ucsd.edu






Hello everyone,

 

We are looking for some standard biological samples to image under negative stained or cryo conditions.  These standards would be used show new TEM users what sort of data they can expect with correctly prepared and handled samples.  I suppose the only requirements for this standard would be MW >250kd, that it stains well with Uranyl Acetate or Uranyl Formate and that it be fairly homogeneous.   

 

Ideally we would like to purchase a standard instead of purify it but I am not having much luck finding what I thought would have been common (GroEL, TMV) TEM standards for sale.  If necessary we will purify our own but I thought maybe someone on this listserv may have more information on how to obtain such a standard. Thank you for your time and help.

 

Best wishes,
Jonathan Remis 

________________________________________







JONATHAN REMIS




CryoEM Facilities Manager 




Northwestern University 

Department of Molecular Biosciences 




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