[3dem] Uranyl Formate powder lifetime
Fabien Alexandre Bergeret, Dr
fabien.bergeret at mcgill.ca
Wed Nov 19 08:14:15 PST 2014
Thank you all of us for these advices.
De : Svetomir B Tzokov [s.b.tzokov at sheffield.ac.uk]
Envoyé : mercredi 19 novembre 2014 11:10
À : 3dem at ncmir.ucsd.edu
Cc : Fabien Alexandre Bergeret, Dr
Objet : Re: [3dem] Uranyl Formate powder lifetime
Currently we use a bottle of Uranyl formate (UF) powder which we believe is about 10 years old - although we can't be sure, because it was inherited from another lab in Sheffield 5 years ago.
The stain is fine.
We only make partially neutralised 0.75% solution of UF in 5 ml aliquots for immediate use. This solution would last normally for up to 1-2 weeks in the fridge. The solution is discarded if the UF starts precipitating.
UF must not be exposed to direct sunlight and should be kept away from any other light whenever possible. We use foil-wrapped beakers/tubes for the stain solution. The powder itself came in a brown bottle and is stored in the dark.
On Wed, 19 Nov 2014 15:10:35 -0000, Fabien Alexandre Bergeret, Dr <fabien.bergeret at mcgill.ca> wrote:
I would like to order Uranyl Formate powder from SPI (U.S.A). SPI company mentions that Uranium metals are subject to special restrictions from U.S.A and must be shipped by air only. The price for shipping is extremely expensive, more than twice the price of the compound itself (for 5g).
I am considering buying multiple vials but I don't know how stable is the Uranyl Formate powder? Can I store it for months, years, ...?
I also noticed on Dr. Grigorief's website that Uranyl Formate solutions can be frozen and stored at -80C. If you have any experience with this, how long Uranyl Formate aliquots can be stored at -80C?
Thanks in advance for your help.
Svetomir B Tzokov
Electron Microscopy Facility Manager
Krebs Institute for Biomolecular Research
Dept Molecular Biology and Biotechnology
The University of Sheffield
Firth Court, Western Bank
Sheffield S10 2TN
Tel: +44 (0)114 222 4674
Fax: +44 (0)114 222 4243
Mob: +44 (0)7905 832358
Email: s.b.tzokov at sheffield.ac.uk
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