[3dem] Uranyl Formate powder lifetime

Svetomir B Tzokov s.b.tzokov at sheffield.ac.uk
Wed Nov 19 08:10:08 PST 2014


Hi Fabien,

Currently we use a bottle of Uranyl formate (UF) powder which we believe  
is about 10 years old - although we can't be sure, because it was  
inherited from another lab in Sheffield 5 years ago.

The stain is fine.

We only make partially neutralised 0.75% solution of UF in 5 ml aliquots  
for immediate use. This solution would last normally for up to 1-2 weeks  
in the fridge. The solution is discarded if the UF starts precipitating.

UF must not be exposed to direct sunlight and should be kept away from any  
other light whenever possible. We use foil-wrapped beakers/tubes for the  
stain solution. The powder itself came in a brown bottle and is stored in  
the dark.

Kind regards,
Svet



On Wed, 19 Nov 2014 15:10:35 -0000, Fabien Alexandre Bergeret, Dr  
<fabien.bergeret at mcgill.ca> wrote:

> Dear all,
> I would like to order Uranyl Formate powder from SPI (U.S.A). SPI  
> company mentions that Uranium metals are subject >to special  
> restrictions from U.S.A and must be shipped by air only. The price for  
> shipping is extremely expensive, >more than twice the price of the  
> compound itself (for 5g).
>
> I am considering buying multiple vials but I don't know how stable is  
> the Uranyl Formate powder? Can I store it for >months, years, ...?I also  
> noticed on Dr. Grigorief's website that Uranyl Formate solutions can be  
> frozen and stored at -80C. If you >have any experience with this, how  
> long Uranyl Formate aliquots can be stored at -80C?
>
> Thanks in advance for your help.
>
> Best,
>
> Fabien Bergeret
> McGill University
> Canada



-- 

Svetomir B Tzokov
Electron Microscopy Facility Manager
Krebs Institute for Biomolecular Research
Dept Molecular Biology and Biotechnology
The University of Sheffield
Firth Court, Western Bank
Sheffield S10 2TN
United Kingdom
Tel: +44 (0)114 222 4674
Fax: +44 (0)114 222 4243
Mob: +44 (0)7905 832358
Email: s.b.tzokov at sheffield.ac.uk


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