[3dem] Virus on Carbon

[Wright, Elizabeth R.]

Hi Luiza,

We optimized the affinity grid methods for capturing enveloped viruses, including HIV, onto EM grids. The paper reference is: Microsc Microanal. 2014 Feb; 20(1): 164-74. This worked very well for many tough to handle viruses.

Liz

----
Elizabeth R. Wright, PhD
Assistant Professor
Emory University
erwrigh at emory.edu<mailto:erwrigh at emory.edu>
Ph: (404) 727-4665

From: Luiza Mendonça <luiza at caltech.edu<mailto:luiza at caltech.edu>>
Date: Thursday, March 13, 2014 7:41 PM
To: 3DEM Mailing List <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] Virus on Carbon

Dear colleagues,

I work with HIV particles and (at least in my samples), they like the carbon film on the grids much better than the holes, but for me this is a big problem, as, even using high titer preps, they rarely go to the holes, resulting in less-than-perfect tomograms. Has anyone else had this problem? What did you do to solve it?
My particles are resusspended in PBS (with gold fiducials) at the time of freezing.

Best,
Luiza.

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