[3dem] Virus on Carbon

[C.J.]

I also came accross problems of something like Luiza's. My sample is a
macromolecule.

Xinghong, whether your method could solve my problem? Thanks.



2014-03-14 12:16 GMT+08:00 Xinghong Dai <bestdz at gmail.com>:

> Hi Luiza,
>
> Maybe you can try to make the ice a little bit thicker to see if that will
> make any difference. They theory is that, if the ice is too thin, big
> particles will not stay in the hole due to size exclusion effect. I had
> similar situation when working with big viruses.
>
> Good luck!
>
>
>
> Xinghong
>
>
>
>
>
> *发件人**:* 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] *代表
> *Luiza Mendon?a
> *发送时间:* Thursday, March 13, 2014 4:41 PM
> *收件人:* 3DEM Mailing List
> *主题:* [3dem] Virus on Carbon
>
>
>
> Dear colleagues,
>
>
>
> I work with HIV particles and (at least in my samples), they like the
> carbon film on the grids much better than the holes, but for me this is a
> big problem, as, even using high titer preps, they rarely go to the holes,
> resulting in less-than-perfect tomograms. Has anyone else had this problem?
> What did you do to solve it?
>
> My particles are resusspended in PBS (with gold fiducials) at the time of
> freezing.
>
>
>
> Best,
>
> Luiza.
>
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>
>


-- 
Regards!
C.J.
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