[3dem] Virus on Carbon

[Xinghong Dai]

Hi Luiza,

Maybe you can try to make the ice a little bit thicker to see if that will make any difference. They theory is that, if the ice is too thin, big particles will not stay in the hole due to size exclusion effect. I had similar situation when working with big viruses.

Good luck!

 

Xinghong 

 

 

发件人: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] 代表 Luiza Mendon?a
发送时间: Thursday, March 13, 2014 4:41 PM
收件人: 3DEM Mailing List
主题: [3dem] Virus on Carbon

 

Dear colleagues,

 

I work with HIV particles and (at least in my samples), they like the carbon film on the grids much better than the holes, but for me this is a big problem, as, even using high titer preps, they rarely go to the holes, resulting in less-than-perfect tomograms. Has anyone else had this problem? What did you do to solve it?

My particles are resusspended in PBS (with gold fiducials) at the time of freezing.

 

Best,

Luiza.

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