[3dem] Relion 1.3: multiple picks of same particle with autopick

Dieter Blaas dieter.blaas at meduniwien.ac.at
Mon Dec 8 22:06:37 PST 2014


Thanks to all:
increasing the distance threshold from 100 A to 120 A eliminated almost 
all multiple picks!
Best, Dieter

------------------------------------------------------------------------
Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: dieter.blaas at meduniwien.ac.at
------------------------------------------------------------------------

Am 08.12.2014 16:39, schrieb rkhayat at ccny.cuny.edu:
> There is a distance threshold that you need to play with to eliminate particles that have their center located within a certain distance from one another. It's described in the tutorial.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY  10031
> Tel. (212) 650-6070
> www.khayatlab.org
>
>
> ---- Original message ----
>> Date: Sat, 06 Dec 2014 08:23:20 +0100
>> From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Dieter Blaas <dieter.blaas at meduniwien.ac.at>)
>> Subject: [3dem] Relion 1.3: multiple picks of same particle with autopick
>> To: 3dem at ucsd.edu
>>
>> Hi Sjors and all,
>>       what might be the reason for getting (amongst correctly picked
>> particles) multiple, slightly shifted picks (coordinates) of the same
>> particle when running autopick in Relion 1.3 (please see the attached
>> example)? How can this be avoided?
>> Thanks for hints, best, Dieter
>>
>> ------------------------------------------------------------------------
>> Dieter Blaas,
>> Max F. Perutz Laboratories
>> Medical University of Vienna,
>> Inst. Med. Biochem., Vienna Biocenter (VBC),
>> Dr. Bohr Gasse 9/3,
>> A-1030 Vienna, Austria,
>> Tel: 0043 1 4277 61630,
>> Fax: 0043 1 4277 9616,
>> e-mail: dieter.blaas at meduniwien.ac.at
>> ------------------------------------------------------------------------
>>
>> ________________
>> picking.jpg (99k bytes)
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