[3dem] Relion 1.3: multiple picks of same particle with autopick

rkhayat at ccny.cuny.edu rkhayat at ccny.cuny.edu
Mon Dec 8 07:39:45 PST 2014

There is a distance threshold that you need to play with to eliminate particles that have their center located within a certain distance from one another. It's described in the tutorial. 

Best wishes,

Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY  10031
Tel. (212) 650-6070

---- Original message ----
>Date: Sat, 06 Dec 2014 08:23:20 +0100
>From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Dieter Blaas <dieter.blaas at meduniwien.ac.at>)
>Subject: [3dem] Relion 1.3: multiple picks of same particle with autopick  
>To: 3dem at ucsd.edu
>Hi Sjors and all,
>      what might be the reason for getting (amongst correctly picked 
>particles) multiple, slightly shifted picks (coordinates) of the same 
>particle when running autopick in Relion 1.3 (please see the attached 
>example)? How can this be avoided?
>Thanks for hints, best, Dieter
>Dieter Blaas,
>Max F. Perutz Laboratories
>Medical University of Vienna,
>Inst. Med. Biochem., Vienna Biocenter (VBC),
>Dr. Bohr Gasse 9/3,
>A-1030 Vienna, Austria,
>Tel: 0043 1 4277 61630,
>Fax: 0043 1 4277 9616,
>e-mail: dieter.blaas at meduniwien.ac.at
>picking.jpg (99k bytes)
>3dem mailing list
>3dem at ncmir.ucsd.edu

More information about the 3dem mailing list