[3dem] Custom Cut Vitrobot Blotting Paper

[Silvestry Ramos, Mariena O]

Hi Kyle,

For the outer hole, I don't know but during my time here at Vandy as a student, we used a hole punch called Marvy (I believe you can find it at craft stores, costs less than $15 or here: http://www.uchida.com/c-1-paper-punches.aspx#Filter=[EntityIDs=@%285%29@*Sort=3*ava=0] ) in the round size. It punched the inner holes with the proper size for the Vitrobot. We have another Vitrobot that uses paper with pre-punched holes from Ted Pella. My two cents, 

Best, 

Mariena



Mariena Silvestry Ramos, PhD
Research Specialist Senior
Center for Structural Biology - CryoEM Lab
Vanderbilt University Medical Center
MCN RR1207
Nashville, TN
ph. 615-322-4671

________________________________________
From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] on behalf of Wim Hagen [hagen at embl.de]
Sent: Tuesday, June 11, 2013 1:55 AM
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Custom Cut Vitrobot Blotting Paper

Dear Kyle,

I suspect Willem used sticky during his last days at EMBL tape for the
Whatman 1 blotting paper typically used, and the EMBL vitrobot had no
clamping rings.
I had trouble working this way using Whatman 597 (used to be Schleicher
& Schuell) and got new clamping rings. Our mechanical shop makes the
holes in the 597 paper.

Best,

Wim Hagen
EMBL Heidelberg

On 11.06.2013 08:04, Willem Tichelaar wrote:
> Dear Kyle
>
>  As far as the inner holes are concerned, do you really need them?
> Can't you use small pieces of double-sided sticking tape to attach the
> paper to the pads? (Can be cut from the kind of pieces of double-sided
> tape that are used to put photographs in an album, for example).
>
>  Best wishes,
>  Willem Tichelaar
>
> On 6/11/13 7:24 AM, Kyle P. Eagen wrote:
>
>> Dear all,
>>
>> Does anyone have experience cutting their own blotting paper for the
>> Vitrobot from square sheets? I have blotting paper I would like to
>> try that only comes as squares and would need to cut both the inner
>> hole and the outer edge so the blotting paper can fit onto the
>> Vitrobot. Any advice would be greatly appreciated.
>>
>> Thanks!
>>
>> Best,
>> Kyle
>>
>> --
>> Kyle P. Eagen
>> M.D./Ph.D. Doctoral Student
>> Medical Scientist Training Program
>> Program in Biophysics
>> Roger Kornberg Laboratory
>> Department of Structural Biology
>> Stanford University School of Medicine
>> 299 Campus Drive West
>> Fairchild Building Room D109
>> Stanford, CA 94305-5126
>> USA
>> Lab Phone: (650) 724-9944
>> keagen at stanford.edu
>>
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>
> --
> Dr. Willem Tichelaar
> Richard-Drach-Str. 5
> 69123 Heidelberg, Deutschland
>
> phone: +49 (0)6221 779409; fax: +49 (0)6221 779411
> e-mail: w.tichelaar at online.de
>
>
>
> Links:
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>
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