[3dem] Custom Cut Vitrobot Blotting Paper

[Wim Hagen]

Dear Kyle,

I suspect Willem used sticky during his last days at EMBL tape for the 
Whatman 1 blotting paper typically used, and the EMBL vitrobot had no 
clamping rings.
I had trouble working this way using Whatman 597 (used to be Schleicher 
& Schuell) and got new clamping rings. Our mechanical shop makes the 
holes in the 597 paper.

Best,

Wim Hagen
EMBL Heidelberg

On 11.06.2013 08:04, Willem Tichelaar wrote:
> Dear Kyle
> 
>  As far as the inner holes are concerned, do you really need them?
> Can't you use small pieces of double-sided sticking tape to attach the
> paper to the pads? (Can be cut from the kind of pieces of double-sided
> tape that are used to put photographs in an album, for example).
> 
>  Best wishes,
>  Willem Tichelaar
> 
> On 6/11/13 7:24 AM, Kyle P. Eagen wrote:
> 
>> Dear all,
>> 
>> Does anyone have experience cutting their own blotting paper for the
>> Vitrobot from square sheets? I have blotting paper I would like to
>> try that only comes as squares and would need to cut both the inner
>> hole and the outer edge so the blotting paper can fit onto the
>> Vitrobot. Any advice would be greatly appreciated.
>> 
>> Thanks!
>> 
>> Best,
>> Kyle
>> 
>> --
>> Kyle P. Eagen
>> M.D./Ph.D. Doctoral Student
>> Medical Scientist Training Program
>> Program in Biophysics
>> Roger Kornberg Laboratory
>> Department of Structural Biology
>> Stanford University School of Medicine
>> 299 Campus Drive West
>> Fairchild Building Room D109
>> Stanford, CA 94305-5126
>> USA
>> Lab Phone: (650) 724-9944
>> keagen at stanford.edu
>> 
>> _______________________________________________
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> 
> --
> Dr. Willem Tichelaar
> Richard-Drach-Str. 5
> 69123 Heidelberg, Deutschland
> 
> phone: +49 (0)6221 779409; fax: +49 (0)6221 779411
> e-mail: w.tichelaar at online.de
> 
> 
> 
> Links:
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> 
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