[3dem] How to determine a symmetry of a ring particle?

[Bernard Heymann]

Dear Alexander 

Another alternative is the method used in:
Ultramicroscopy. 1995 Sep;60(2):219-28

It can handle polymorphism.

You can find the application here:
http://lsbr.niams.nih.gov/lsbr/RotaStat.html

Best

Bernard

--------------------------------------------------------------------------------------------------------------------
J. Bernard Heymann Ph.D.
LSBR/NIAMS/NIH
Rm 1515, 50 South Dr, NIH
Bethesda MD 20892-8025
Tel. 301-451-8241




On Jan 14, 2013, at 4:30 AM, Philip Köck <Philip.Koeck at ki.se> wrote:

> That’s another good idea.
> You might actually have eigen images already. They could be one of the outputs of the 2D classification
> you did in Eman.
> Look at Dube et al. to learn how to interpret the eigen images, if you find them.
>  
> From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of michael
> Sent: 14 January 2013 10:23
> To: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] How to determine a symmetry of a ring particle?
>  
> Dear Alexander,
> 
> Dube et al. 1993 are using multivariate statistical analysis (MSA) to estimate the symmetry properties by looking at the eigenimages.
> 
> A description of the method can be found here: www.singleparticles.org/methodology . You can download the PDF file "Multivariate Statistical Analysis in Single Particle (Cryo) Electron Microscopy". The related chapter is "12. MSA symmetry analysis".
> 
> The original paper is: Dube P, Tavares P, Lurz R and van Heel M EMBO J. (1993) 1303-1309.
> 
> I also want to refer to Pawel's hint: the method is a symmetry estimation and not a determination.
> 
> Regards
> Michael
> 
> 
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