[3dem] How to determine a symmetry of a ring particle?
Philip.Koeck at ki.se
Mon Jan 14 01:30:44 PST 2013
That's another good idea.
You might actually have eigen images already. They could be one of the outputs of the 2D classification
you did in Eman.
Look at Dube et al. to learn how to interpret the eigen images, if you find them.
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of michael
Sent: 14 January 2013 10:23
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] How to determine a symmetry of a ring particle?
Dube et al. 1993 are using multivariate statistical analysis (MSA) to estimate the symmetry properties by looking at the eigenimages.
A description of the method can be found here: www.singleparticles.org/methodology<http://www.singleparticles.org/methodology> . You can download the PDF file "Multivariate Statistical Analysis in Single Particle (Cryo) Electron Microscopy". The related chapter is "12. MSA symmetry analysis".
The original paper is: Dube P, Tavares P, Lurz R and van Heel M EMBO J. (1993) 1303-1309.
I also want to refer to Pawel's hint: the method is a symmetry estimation and not a determination.
Image Science Software GmbH
D - 14193 Berlin
Besuchereingang / Entrance:
Tel: +49 30 8909 5326
Fax: +49 30 8909 5321
URL: www. ImageScience .de
HRB: 33106 Berlin-Charlottenburg
VAT/USt-Id-Nr. DE 136613484
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the 3dem