[3dem] Vitrobot Mark IV water cylinder

Bob Grassucci rg2502 at columbia.edu
Wed Dec 18 14:21:32 PST 2013

Hi All,
Thanks for all of your helpful suggestions.  Below is what I have tried 
and my conclusions.
1 There is no leak in the water cylinder that would cause it to empty.
2 The sonicator is working (I took off the cylinder and sprayed myself 
with water.
3 I can hear the fan so it should be dispersing the water vapor.
4 I can check the humidity sensor but doubt that is the problem since it 
gets there at times.
5 I set the humidifier to manual and got little puffs of humidity rather 
than blowing like crazy.
6. This is at 6 degrees so it does take a while to bring it to 100% 
since the humidity condenses on the cold surfaces.
7 When I mount the cylinder and pull with the syringe I get very little 
resistance making me suspect a poor seal with the chamber thus not 
pulling water from the outer to inner vessel.

Thanks again for your help.

On 12/18/2013 11:47 AM, Bob Grassucci wrote:
> Hi All,
> We are having a problem getting the humidity up to 100% on our 
> Vitrobot Mark IV and expect it might have something to do with the 
> water cylinder or it's seal to where it is mounted.  The water 
> container seems a bit looser than what I remember and suspect this may 
> have something to do with the humidity problem.  My question to the 
> list is has anyone else had this problem and if so how did you solve 
> it?  Thanks.
> Bob
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