[3dem] prepare Formvar Grids

[Cristina Berciu]

Hi,
I used Formvar film but a lower concentration (0.25% in ethylene
dichloride) and it works always I use it. Definitely it will work for your
higher concentration.

I clean the glass slides with only acetone (99. %) and I clean them only
with optical lens tissue, to prevent anything leftover on the glass slide.
Usually I pour a little acetone on the glass and clean it with the tissues.
I do it twice, and i keep cleaning with the tissues till I feel that the
tissue slides easily on the surface of the glass. I leave the clean glass
slide for ~2 minutes, to be sure it is completely dry, then I dip it (along
it s length) in the jar with Formvar solution. Immediately I lift it up and
I touch the lower edge to a stack of 5-6 kimwipes on top of each other. You
can notice how the kimwipes suck the extra solution. Then move it in a
different spot and another one, and keep the slide for a few seconds on
each one, till you notice that NO wet mark on the kimwipes is left. Then
lean the glss slide  against something for a couple of minutes. Then cut
with the razor blade the Formvar on one side of the glass slide (bottom,
up, and sides) and dip the glassslide in the dd H2O in an angle of 45
degrees, slowly. It comes very nicely.

Good luck!

Cristina

On Thu, Sep 27, 2012 at 4:30 PM, Denis Chaix <denis.chaix at ibs.fr> wrote:

> Hello
> We have actualy some troubles to prepare Formvar films.
> In fact it's impossible to release the film from the glass slide.
> Here you can find our protocol
>
> Formvar solution. 0,4% in chloroform .
> Pour the solution into a clean Coplin jar
> Dip pre-cleaned slides ( rub thoroughly with Kimwipes) into beaker.
> Drain corner on side of beaker.
> Blot edge of slide on filter paper.
> Score the edges of the Formvar film with an acetone-cleaned razor blade.
> Breathe over the length of the slide to loosen the film.
> Float the film off onto a clean water surface by slowly immersing the
> slide into the water at a 45° angle.
>
>
> Have you some tricks or tips to improve this protocol and help the release
> of the film ?
> For example, have you some ideas like special brand of slides, or a way to
> clean the slides.
>
> Thanks
>
>
>
> Denis
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