[3dem] prepare Formvar Grids

[Cristina Berciu]

Yes, it happens sometimes the slides in a box to  make it more difficult.

On Thu, Sep 27, 2012 at 5:36 PM, Cristina Berciu <cberciu at brandeis.edu>wrote:

> Hi,
> I used Formvar film but a lower concentration (0.25% in ethylene
> dichloride) and it works always I use it. Definitely it will work for your
> higher concentration.
>
> I clean the glass slides with only acetone (99. %) and I clean them only
> with optical lens tissue, to prevent anything leftover on the glass slide.
> Usually I pour a little acetone on the glass and clean it with the tissues.
> I do it twice, and i keep cleaning with the tissues till I feel that the
> tissue slides easily on the surface of the glass. I leave the clean glass
> slide for ~2 minutes, to be sure it is completely dry, then I dip it (along
> it s length) in the jar with Formvar solution. Immediately I lift it up and
> I touch the lower edge to a stack of 5-6 kimwipes on top of each other. You
> can notice how the kimwipes suck the extra solution. Then move it in a
> different spot and another one, and keep the slide for a few seconds on
> each one, till you notice that NO wet mark on the kimwipes is left. Then
> lean the glss slide  against something for a couple of minutes. Then cut
> with the razor blade the Formvar on one side of the glass slide (bottom,
> up, and sides) and dip the glassslide in the dd H2O in an angle of 45
> degrees, slowly. It comes very nicely.
>
> Good luck!
>
> Cristina
>
>  On Thu, Sep 27, 2012 at 4:30 PM, Denis Chaix <denis.chaix at ibs.fr> wrote:
>
>> Hello
>> We have actualy some troubles to prepare Formvar films.
>> In fact it's impossible to release the film from the glass slide.
>> Here you can find our protocol
>>
>> Formvar solution. 0,4% in chloroform .
>> Pour the solution into a clean Coplin jar
>> Dip pre-cleaned slides ( rub thoroughly with Kimwipes) into beaker.
>> Drain corner on side of beaker.
>> Blot edge of slide on filter paper.
>> Score the edges of the Formvar film with an acetone-cleaned razor blade.
>> Breathe over the length of the slide to loosen the film.
>> Float the film off onto a clean water surface by slowly immersing the
>> slide into the water at a 45° angle.
>>
>>
>> Have you some tricks or tips to improve this protocol and help the
>> release of the film ?
>> For example, have you some ideas like special brand of slides, or a way
>> to clean the slides.
>>
>> Thanks
>>
>>
>>
>> Denis
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