[3dem] Gatan and Leica

[Anindito Sen]

Hi Ed,

I have been using Leica Cryo GP regularly  in our lab. This instrument blots the sample loaded on the grid only from one side but does its job in a clean manner. The blot force can be set according the user's choice and doesnot break the carbon (depending on the setting). 

Moreover, the user can set the position of the filter paper and the grid in the vertical direction  to get the best blot. 

One of the advantage that I find is the ease of "loading" the Ethane in this instrument than in Vitrobot. 

Some minor instrument errors like " Z axis motor error" appears appears on the display and   the instrument seize  to function and needs a complete restart. However, this occurs  occasionally. 

Hope this helps.


Best Wishes

 
Dr. Anindito Sen (Ph.D)
>Department of Cell Biology & Anatomy
>Graduate School of Medicine
>University of Tokyo
>Tel & fax: +81-3-5841-3339



________________________________
 From: Edward Egelman <egelman at virginia.edu>
To: 3DEM Mailing List <3dem at ncmir.ucsd.edu> 
Sent: Saturday, 15 September 2012, 9:05
Subject: [3dem] Gatan and Leica
 

Hi,
  We have been using the Vitrobot, but have had numerous problems.
    Does anyone have experience with the Leica or Gatan cryo-plungers?
Regards,
Ed


-- 


  


Edward H. Egelman, Ph.D.
Professor
Dept. of Biochemistry and Molecular Genetics 
University of Virginia 


phone: 434-924-8210 
fax: 434-924-5069 
egelman at virginia.edu 
http://www.people.virginia.edu/~ehe2n 





  


Edward H. Egelman, Ph.D.
Professor
Dept. of Biochemistry and Molecular Genetics 
University of Virginia 


Editor-in-Chief
Biophysical Journal
phone: 434-924-8210 
fax: 434-924-5069 
egelman at virginia.edu 
http://www.people.virginia.edu/~ehe2n 

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