[3dem] CCD retracted when emptying buffer tank

[Jamonshi]

Hi Wim,

As I can recall, Tecnai 3.x does not retract CCD when buffer cycle runs. Can you be more specific what version you have? And what type of acquisition software you are referring to? 

To answer Rich, this is our T12, with Ultrascan4000 and Orius, software is Tecnai 4.2 and DM 1.8.3. I am using UCSF tomo to collect data.

Regards,
Jian SHI
Manager, cryoEM facility
Center for BioImaging Science
National University of Singapore

On Jun 15, 2012, at 12:24 AM, Wim Hagen <wim.hagen at embl.de> wrote:

> Hi Jian,
> 
> On all my FEI instruments, the software for the retractable Gatan cameras automatically inserts the camera when it is retracted and an acquisition is taken, so I don't have any problems if a camera get's retracted and not inserted, I actually don't even know if the CCD retraction is happening as my acquisition simply runs, including buffer cycles.
> 
> Best regards,
> 
> Wim
> 
> On Jun 14, 2012, at 6:05 PM, j s wrote:
> 
>> Dear Lister,
>>  
>> Our FEI T12 received a software upgrade to Tecnai 4.2. Coming with it there is a unexpected 'feature' (according to FEI): The CCD camera is retracted when the emptying buffer tank start to run, but NOT reinserted afterwards. Such behaviour totally defeats any intention of running unsupervised automatic data collection, including FEI's own software. I am wondering if anyone else had similar experience and how to get around of it. Your help is appreciated.
>>  
>> Regards,
>>  
>> Jian SHI
>> NUS-CBIS
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>> 3dem at ncmir.ucsd.edu
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> 
> -------------------------------------------------------------------
>   Wim Hagen
>   Senior Engineer Electron Microscopy
>   European Molecular Biology Laboratory (EMBL)
>   Heidelberg, Germany                    
> --------------------------------------------------------------------
> 
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