[3dem] Double labeling one protein

Leandro Lemgruber llemgruber at gmail.com
Tue Feb 28 05:53:31 PST 2012


Dear members of the list,

I have a question that I hope someone can help me with.
I was planning to labeled the C-terminal and the N-terminal of a protein,
both would be labeled with different primary antibodies.
Do you think is possible to use primary+secondary (coupled to gold)
antibodies?

Thanks for the help

Best

Leandro
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