[3dem] fiducial markers for cryoTEM

[Marco Sergio]

Dear Frank,

                If you want to perform tomography skipping the use of fiducials you may also try using software that performs reconstructions using image alignment based on automatic points recognition such as TomoJ (we developed in our lab).
See the following papers for details:

1.       Sorzano, C.O., Messaoudi, C., Eibauer, M., Hegerl, R., Nickell, S., J.R. Bilbao-Castro., Marco, S., Carazo, J.M. (2009). Marker-free image registration of electron tomography tilt-series. BMC Bioinformatics. 10:124-135

2.       Messaoudi, C., Boudier, T., Sorzano, C.O.S., Marco, S. (2007). TomoJ: tomography software for 3D reconstruction in transmission electron microscopy.  BMC Bioinformatics. 8:288-297.

3.  Manual, tutorial and software : http://u759.curie.fr/fr/telechargements/softwares/tomoj/tomoj-00733



You have Three different possible algorithms grids, local minima and local maxima. Sometimes several trials for automatic seed detection are required.



If you decide to try and need help do not hesitate to contact me or Cedric messaoudi (cedric.messaoudi at curie.fr)










Sergio Marco

Research Director INSERM
Institut Curie / INSERM U759
Campus Universitaire d'Orsay, Bat 112
91405 Orsay cedex FRANCE

Phone +33 1 69 86 30 77
Fax +33 1 69 07 53 27

sergio.marco at curie.fr<mailto:sergio.marco at curie.fr>


http://u759.curie.fr/en/groups/structural-bases-cellular-processes/structural-bases-cellular-processes-00308
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De : 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] De la part de Frank Polzer
Envoyé : mercredi 22 février 2012 12:49
À : 3dem at ncmir.ucsd.edu
Objet : [3dem] fiducial markers for cryoTEM

Dear all,

Does anyone know a good alternative for fiducial markers if gold fiducials  (e.g., Aurion(tm) Gold Sol, 10 nm) heavily adsorbs to the sample. In this case, I have synthetic block-co-polymer micelles (in water) which exhibits a quaternized 2-vinylpyridine groups, so that the gold fiducial markers are strongly correlated within the micellar structure (I expect due to the positive charge of the quaternized 2-vinylpyridine) and almost no gold nanoparticles can be found apart from the sample within the vitreous film.

Thank you all in advance for your help!

Kind regards,

Frank

Frank Polzer
TEM Group
Institute of Physics
Humboldt Universität zu Berlin
Newtonstraße 15
12489 - Berlin

Tel.: +49 30 2093-7905 (office)
Tel.: +49 30 2093-7829 (TEM)
Fax: +49 30 2093-7760

frank.polzer at physik.hu-berlin.de

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