[3dem] fiducial markers for cryoTEM

Guenter Resch guenter.resch at imba.oeaw.ac.at
Wed Feb 22 09:46:48 PST 2012


Hi Frank,

> Does anyone know a good alternative for fiducial markers if gold
> fiducials (e.g., Aurion™ Gold Sol, 10 nm) heavily adsorbs to the
> sample. 

I'd suggest to give protein coated gold particles a try - this should
eliminate the surface charge of the golds and hence decrease adsorption.

You can coat the gold sol either yourself (e.g. with BSA, see Urban E.,
et al. (2010), Nature Cell Biology) or purchase coated gold particles
from Aurion et al.

Best,

	Guenter

-- 
Dr. Guenter Resch
Head of Electron Microscopy, Campus Science Support Facilities GmbH
Phone: +43 (1) 79044-4250; Fax: -224250; Web: http://www.csf.ac.at
Post: Dr. Bohr-Gasse 3, 1030 Vienna, Austria, European Union


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