[3dem] fiducial markers for cryoTEM

[Frank Polzer]

Dear all,

 

Does anyone know a good alternative for fiducial markers if gold fiducials
(e.g., Aurion™ Gold Sol, 10 nm) heavily adsorbs to the sample. In this case,
I have synthetic block-co-polymer micelles (in water) which exhibits a
quaternized 2-vinylpyridine groups, so that the gold fiducial markers are
strongly correlated within the micellar structure (I expect due to the
positive charge of the quaternized 2-vinylpyridine) and almost no gold
nanoparticles can be found apart from the sample within the vitreous film. 

 

Thank you all in advance for your help!

 

Kind regards,

 

Frank

 

Frank Polzer

TEM Group

Institute of Physics

Humboldt Universität zu Berlin

Newtonstraße 15

12489 - Berlin

 

Tel.: +49 30 2093-7905 (office)

Tel.: +49 30 2093-7829 (TEM)

Fax: +49 30 2093-7760

 

frank.polzer at physik.hu-berlin.de 

 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20120222/5c3b1c44/attachment.html