[3dem] C-flats

[Juha Huiskonen]

Dear Thorsten,

there is a version of C-flats which has got a thicker carbon. We have had good experiences with CF-2/1/2C-T. In our hands these are less fragile than the normal ones.

If there is frozen ethane on the grid after plunging, 
I suspect that the carbon layer may break when this layer detaches from the grid during transfer to a grid storage box. To prevent ethane freezing on the grid, we use a mixture of ethane and propane with good results.

I have no experience on Vitrobot, however, we use Gatan CP3.

Best wishes,
Juha

-- 
Dr. Juha T. Huiskonen
Group leader, Academy of Finland Research Fellow
Oxford Particle Imaging Centre
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
University of Oxford
Roosevelt Drive, Oxford OX3 7BN

http://www.strubi.ox.ac.uk/strubi/research/huiskonen/
Email: juha at strubi.ox.ac.uk
Tel.: +44 1865 287 844
Mob.: +44 7576 505 422