[3dem] C-flats

[Gérard Pehau-Arnaudet]

Dear Thorsten
I have observed the same problem than you and i am now using Quantifoil R2/2
with sucess.
Best regards
Gérard

2011/5/25 Thorsten Mielke <mielke at molgen.mpg.de>

> Dear all,
>
> we are trying to vitrify some small proteins on c-flat grids (type
> CF-2/4-4C) using a Vitrobot from FEI. However, after plunge-freezing, the
> carbon film is heavily destroyed. Additionally, we find only a few good
> holes on the remaining intact meshes. The carbon film also seems to be
> extremely fragile and easily disrupts upon focusing (300 kV, 155kx Polara).
> The grids seem to be OK (with or without glow-discharching) until
> plunge-freezing. We varied the blotting offset at the Vitrobot to play with
> the blotting force, no success.  Did you observe similar problems with
> C-flats? Any idea how to prevent the carbon film from breaking?
>
> Thanks
>
> Thorsten
>
>
>
>
> --
>
> *******************************************
> Dr. Thorsten Mielke
> - UltraStrukturNetzwerk -
>
> Max Planck Institute for Molecular Genetics
> Ihnestr. 63-73
> D-14195 Berlin
>
> Tel.: +49-(0)30 8413-1644/1141
> Fax: +49-(0)30 8413-1385
> E-mail: mielke at molgen.mpg.de
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20110525/ffa864e7/attachment.html