[3DEM]asymmetric unit segmentation

[C.J.]

Hi, all,

I try to segment a icosahedral virus volume produced by EMAN via
Chimera "Segment Map".
Unexpectedly, the volume was not segmented according icosahedral symmetry.
I want your help whether there is a solution to this problem?

Regards!
C.J.

2011/7/14 C.J. <biocjh at gmail.com>:
> thank you for useful information
>
> regars!
> C.J.
>
> 2011/7/13 Steven Ludtke <sludtke at bcm.edu>:
>> While Bajaj's group at UT Austin has some methods they claim to work fully automatically, segmentation almost always
>> requires some human effort (and additional knowledge) to perform correctly.  I would suggest the 'Segger' tool developed
>> by Grig Pintilie, now built into Chimera, which is only semi-automatic, but easy to use. Chimera has a
>> variety of other useful tools for dealing with symmetry as well, which are at least somewhat documented in the short
>> videos Tom Goddard put together : http://www.cgl.ucsf.edu/chimera/videodoc/videodoc.html
>>
>> ----------------------------------------------------------------------------
>> Steven Ludtke, Ph.D.
>> Associate Professor
>> Co-Director National Center For Macromolecular Imaging
>> Dept of Biochemistry and Mol. Biol.
>> Baylor College of Medicine
>> sludtke at bcm.edu
>> stevel at alumni.caltech.edu
>>
>>
>>
>>
>> On Jul 13, 2011, at 7:39 AM, C.J. wrote:
>>
>>> Dear all,
>>>
>>> Any one have a experience on segmentation of asymmetric unit from
>>> icosahedron virus structure volume?
>>> I wonder whether there is any programme could process that automatically?
>>> Any comment would be appreciated!
>>>
>>> --
>>> Regards!
>>> C.J.
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>>
>>
>
>
>
> --
> Regards!
> C.J.
>



-- 
Regards!
C.J.