[3dem] Re: CEMOVIS

[Yi, Hong]

Thank you, Sergey.  I talked to you at last year's M&M meeting.  I am one
of the two authors on the original SPRF paper (Leunissen JL, Yi H.
Self-pressurized rapid freezing (SPRF): a novel cryofixation method for
specimen preparation in electron microscopy. J Microsc. 2009
Jul;235(1):25-35).  I am very glad that you were able to apply this method
to sample freezing for CEMOVIS. For training purpose, we are trying to
freeze samples for CEMOVIS using different methods.

Hope to talk to you again at Nashville meeting.

Hong

On 7/21/11 4:38 PM, "Sergey Yakovlev" <sergey.lbl at gmail.com> wrote:

>Hi Hong,
>
>You certainly can freeze samples in a planchet for CEMOVIS. It may need
>more cryoprotectant than freezing in capillary.
>
>Alternatively you can use Self Pressure Freezing technique that does not
>need high pressure freezing machine at all. Put the sample (with
>cryoprotectant) in standard HPF copper capillary tube, seal both ends
>with pliers and plunge the capillary into a cryogen. It is easy and
>provides better cryopreservation than conventional HPF equipment.
>
>We have submitted the paper on this technique in Journal of Microscopy
>but it is not available yet. You can meet me at M&M 2011 if you need more
>details.  
>
>Regards,
>Sergey
>
>----------------------------------
>Sergey Yakovlev
>Downing Lab
>Lawrence Berkeley 
>National Laboratory
>One Cyclotron Road
>Mailstop: 1-116
>Berkeley, CA 94720
>mailto:syakovlev at lbl.gov
>
>
>Date: Thu, 21 Jul 2011 17:25:45 +0000
>From: "Yi, Hong" <hyi at emory.edu>
>Subject: [3dem] CEMOVIS
>To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
>Message-ID: <CA4DD950.EA9C%hyi at emory.edu>
>Content-Type: text/plain; charset="us-ascii"
>
>Dear All:
>
>As far as I know, people usually freeze samples in a copper tube when
>doing CEMOVIS.  We have a RMC HPM 010 so can not use a tube holder.  If
>we freeze samples in a planchet, can it be used for CEMOVIS?  If yes, how
>should we mount samples on our Leica UC6/FC6?
>
>Thank you all for your help.
>
>Hong
>
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>cedric
>
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