[3dem] Re: CEMOVIS

[Sergey Yakovlev]

Hi Hong,

You certainly can freeze samples in a planchet for CEMOVIS. It may need more cryoprotectant than freezing in capillary.

Alternatively you can use Self Pressure Freezing technique that does not need high pressure freezing machine at all. Put the sample (with cryoprotectant) in standard HPF copper capillary tube, seal both ends with pliers and plunge the capillary into a cryogen. It is easy and provides better cryopreservation than conventional HPF equipment. 

We have submitted the paper on this technique in Journal of Microscopy but it is not available yet. You can meet me at M&M 2011 if you need more details.  

Regards,
Sergey

----------------------------------
Sergey Yakovlev
Downing Lab
Lawrence Berkeley 
National Laboratory
One Cyclotron Road
Mailstop: 1-116
Berkeley, CA 94720
mailto:syakovlev at lbl.gov


Date: Thu, 21 Jul 2011 17:25:45 +0000
From: "Yi, Hong" <hyi at emory.edu>
Subject: [3dem] CEMOVIS
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Message-ID: <CA4DD950.EA9C%hyi at emory.edu>
Content-Type: text/plain; charset="us-ascii"

Dear All:

As far as I know, people usually freeze samples in a copper tube when doing CEMOVIS.  We have a RMC HPM 010 so can not use a tube holder.  If we freeze samples in a planchet, can it be used for CEMOVIS?  If yes, how should we mount samples on our Leica UC6/FC6?

Thank you all for your help.

Hong

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cedric