[3dem] tilt cryoEM goniometer before sample holder insertion

[David Gene Morgan]

Hi,

	You can even make a spot on the counter top so that you know exactly 
where to put the beaker...

On 04/03/2011 09:33 AM, Sacha De Carlo wrote:
> Hi,
>
> just use a beaker that is clean and dry to collect the liq. nitrogen, insert and rotate the holder back in its vertical position, pour the clean liq, nitrogen back in the holder.
>
> Safe, simple and clean.
>
> Best,
> Sacha
>
>
>
> =============================
>
> Dr. Sacha De Carlo
>
> Sr. Res. Scientist
>
> FEI Company
>
> --- On Sat, 4/2/11, Jingchuan Sun<jingchuan at gmail.com>  wrote:
>
> From: Jingchuan Sun<jingchuan at gmail.com>
> Subject: [3dem] tilt cryoEM goniometer before sample holder insertion
> To: 3dem at ncmir.ucsd.edu
> Date: Saturday, April 2, 2011, 11:36 PM
>
> Hello,Before cryoEM sample holder insertion, it is rotated 90 degree, so most liquid N2 is poured toward user from dewar. I heard that new TEM goniometer could be tilted before holder insertion. Anyone try it with older TEM?
>
> I'm thinking two ways for our JEOL2010F.1) tilt gonimeter +60 degree, insert sample holder (dewar is -30 degree), flip airlock switch to "PUMP", after green light is on and wait for another ~5 min, turn the holder clockwise ~30 degree (now dewar is straight up), tilt gonimeter back to +30 degree, then turn the holder clockwise another ~60 degree (now dewar is +30 degree right), and finally tilt gonimeter back to 0 degree (now dewar is straight up).
>
> 2) tilt gonimeter +45 degree, insert sample (now dewar is -45 degreet), flip airlock to "PUMP", after greenlight is on and wait for ~5 min, turn the holder ~30 degree (now dewar is -15 degree left) and another ~60 degree (now dewar is -45 degree), finally tilt gonimeter back to 0 degree.
>
> The first method need tilt gonimeter two times at high angle, but the holder's dewar is only rotated within 30 degree.
> Anyone try it on JEOL2010F? If it works, it reduce one hazardous operation in cryoEM.
> Thanks!Jim
>
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>
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-- 
                  David Gene Morgan
                   cryoEM Facility
                   320C Simon Hall
            Indiana University Bloomington
                812 856 1457 (office)
                812 856 3221 (EM lab)
             http://bio.indiana.edu/~cryo