[3dem] tilt cryoEM goniometer before sample holder insertion
Sacha De Carlo
sachadecarlo at yahoo.com
Sun Apr 3 06:33:14 PDT 2011
just use a beaker that is clean and dry to collect the liq. nitrogen, insert and rotate the holder back in its vertical position, pour the clean liq, nitrogen back in the holder.
Safe, simple and clean.
Dr. Sacha De Carlo
Sr. Res. Scientist
--- On Sat, 4/2/11, Jingchuan Sun <jingchuan at gmail.com> wrote:
From: Jingchuan Sun <jingchuan at gmail.com>
Subject: [3dem] tilt cryoEM goniometer before sample holder insertion
To: 3dem at ncmir.ucsd.edu
Date: Saturday, April 2, 2011, 11:36 PM
Hello,Before cryoEM sample holder insertion, it is rotated 90 degree, so most liquid N2 is poured toward user from dewar. I heard that new TEM goniometer could be tilted before holder insertion. Anyone try it with older TEM?
I'm thinking two ways for our JEOL2010F.1) tilt gonimeter +60 degree, insert sample holder (dewar is -30 degree), flip airlock switch to "PUMP", after green light is on and wait for another ~5 min, turn the holder clockwise ~30 degree (now dewar is straight up), tilt gonimeter back to +30 degree, then turn the holder clockwise another ~60 degree (now dewar is +30 degree right), and finally tilt gonimeter back to 0 degree (now dewar is straight up).
2) tilt gonimeter +45 degree, insert sample (now dewar is -45 degreet), flip airlock to "PUMP", after greenlight is on and wait for ~5 min, turn the holder ~30 degree (now dewar is -15 degree left) and another ~60 degree (now dewar is -45 degree), finally tilt gonimeter back to 0 degree.
The first method need tilt gonimeter two times at high angle, but the holder's dewar is only rotated within 30 degree.
Anyone try it on JEOL2010F? If it works, it reduce one hazardous operation in cryoEM.
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