[3dem] Re: floating EM grids

[Cheri Hampton]

I, too, plasma clean and then poly-L-lysine coat them. I have not had any problems with floating. 
Cheri

Sent from my iPhone

On Sep 9, 2010, at 3:00 PM, 3dem-request at ncmir.ucsd.edu wrote:

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>   1. Re: floating EM grids (Vladan Lucic)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Thu, 9 Sep 2010 15:25:02 +0200
> From: Vladan Lucic <vladan at biochem.mpg.de>
> Subject: Re: [3dem] floating EM grids
> To: 3dem at ncmir.ucsd.edu
> Message-ID: <201009091525.03207.vladan at biochem.mpg.de>
> Content-Type: Text/Plain;  charset="iso-8859-6"
> 
> 
> Hi Roman,
> 
> We wash grids in EtOH (for 10 min or so), coat with polylysine, wash in H2O 
> and then transfer them to culture dishes. This procedure works fine for us 
> (Quantifoils or lacies, typically NH2A finders). Floating during transport 
> happens very rarely.
> 
> Hope this helps,
> 
> Vladan
> 
> Vladan Lucic, PhD
> Max Planck Institute for Biochemistry
> Am Klopferspitz 18
> D-82152 Martinsried, Germany
> 
> Phone: +49 89 8578-2647
> Fax: +49 89 8578-2641
> 
> 
> On Wednesday 08 September 2010 11:41:27 R.I.Koning at lumc.nl wrote:
>> Dear all,
>> 
>> We regularly grow cells on EM grids and have the problem that these
>> grids have the tendency to float during culture or transport.
>> Does anybody have similar experience and more important do you know any
>> tricks to keep these grids on the bottom?
>> 
>> Best regards,
>> 
>> Roman
>> 
>> Roman I. Koning, Ph.D.
>> Leiden University Medical Center
>> Department of Cell Biology
>> Section Electron Microscopy
>> Postal Zone S1-P
>> P.O.Box 9600
>> 2300 RC, Leiden
>> The Netherlands
>> 
>> visiting adress:
>> Einthovenweg 20,
>> Building 2, zone S1-P, room R-90-20
>> 
>> tel (31) 71 526 9296
>> fax (+31) 71 526 8270
>> r.i.koning at lumc.nl
> 
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