[3dem] floating EM grids

[Vladan Lucic]

Hi Roman,

We wash grids in EtOH (for 10 min or so), coat with polylysine, wash in H2O 
and then transfer them to culture dishes. This procedure works fine for us 
(Quantifoils or lacies, typically NH2A finders). Floating during transport 
happens very rarely.

Hope this helps,

Vladan

Vladan Lucic, PhD
Max Planck Institute for Biochemistry
Am Klopferspitz 18
D-82152 Martinsried, Germany

Phone: +49 89 8578-2647
Fax: +49 89 8578-2641
 

On Wednesday 08 September 2010 11:41:27 R.I.Koning at lumc.nl wrote:
> Dear all,
> 
> We regularly grow cells on EM grids and have the problem that these
> grids have the tendency to float during culture or transport.
> Does anybody have similar experience and more important do you know any
> tricks to keep these grids on the bottom?
> 
> Best regards,
> 
> Roman
> 
> Roman I. Koning, Ph.D.
> Leiden University Medical Center
> Department of Cell Biology
> Section Electron Microscopy
> Postal Zone S1-P
> P.O.Box 9600
> 2300 RC, Leiden
> The Netherlands
> 
> visiting adress:
> Einthovenweg 20,
> Building 2, zone S1-P, room R-90-20
> 
> tel (31) 71 526 9296
> fax (+31) 71 526 8270
> r.i.koning at lumc.nl