[3dem] RE: 3dem Digest, Vol 30, Issue 13

David Gil dcarton at cicbiogune.es
Thu Mar 4 08:43:36 PST 2010

Dear all,

At CIC bioGUNE we have been working with the VITROBOT Model Mk3 since
2007 and these years we have broken three black plastic coolant
containers. As you said in spite of the care and the good use of the
containers we experience some initial cracks that grow making the whole
device useless. 

Finally in April 2009 we receive our fourth new ethane container
(FEI0322S with the HS code 7311001090), done of a different material
(similar to the polyethylene (AmE)). Since then we have not had any
problem. In this material it is impossible that cracks are created.

I hope this information should be useful. Kind wishes,


Electron Microscopy Platform 
Structural Biology Building, CIC bioGUNE 

-----Mensaje original-----
De: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] En
nombre de 3dem-request at ncmir.ucsd.edu
Enviado el: lunes, 22 de febrero de 2010 21:00
Para: 3dem at ncmir.ucsd.edu
Asunto: 3dem Digest, Vol 30, Issue 13

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Today's Topics:

   1. Coolant Container of FEI Vitrobot (Tillmann Pape)


Message: 1
Date: Mon, 22 Feb 2010 17:08:40 +0000
From: Tillmann Pape <t.pape at imperial.ac.uk>
Subject: [3dem] Coolant Container of FEI Vitrobot
To: 3dem at ncmir.ucsd.edu
Message-ID: <70B10B97-3FF5-4A86-99AA-5853F06A355A at imperial.ac.uk>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed

Dear all,

I would like to enquire within the community about their experience  
with the black plastic coolant container of the FEI Vitrobot.

We (with a Vitrobot Model Mark III) have not very good experience  
concerning their lifetime. Again and again we experience very quickly  
some initial cracks upon cooling that grow quite quickly within a few  
months to a sizeable leak of liquid N2, making the whole device not  
very user-friendly anymore and as well a bit of an safety hazard,  
especially for less experienced users. Moreover, the replacement  
prize is quite high.

I was wondering if anybody has had similar experiences and I am  
curious of how they have dealt with this situation.

Many thanks in advance for your feedback.

Kind wishes,


EM Centre
Imperial College London


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End of 3dem Digest, Vol 30, Issue 13




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