[3dem] non aqueous cryoem

Norm Olson nholson at ucsd.edu
Wed Jan 27 12:55:36 PST 2010

There is a user in my facility that wants to plunge freeze liposomes 
for cryoem.  The problem is that the solvent composition is not 
aqueous.  It is 33% water, 11% ethanol, 14% glycerol, and 42%
propylene glycol.    Has anyone ever tried something like this or is 
it even possible?  My first attempts didn't work.


Norm Olson

Norm Olson
Cryoelectron Microscopy Facilities Manager
1510 Bonner Hall
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson at ucsd.edu
Cell:  (858)220-2183
(858)822-6718 - Office; (858)534-5846 - Fax

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