[3dem] non aqueous cryoem

Norm Olson nholson at ucsd.edu
Wed Jan 27 12:55:36 PST 2010


-- 
There is a user in my facility that wants to plunge freeze liposomes 
for cryoem.  The problem is that the solvent composition is not 
aqueous.  It is 33% water, 11% ethanol, 14% glycerol, and 42%
propylene glycol.    Has anyone ever tried something like this or is 
it even possible?  My first attempts didn't work.

Thanks

Norm Olson


______________________________________________________________
Norm Olson
Cryoelectron Microscopy Facilities Manager
1510 Bonner Hall
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson at ucsd.edu
http://cryoem.ucsd.edu
Cell:  (858)220-2183
(858)822-6718 - Office; (858)534-5846 - Fax
______________________________________________________________


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