[3dem] non aqueous cryoem

[Masahide Kikkawa]

Hi,

 I don't have the experience of freezing samples in ethanol or propylene glycol, but if solvent contains >10% glycerol, samples are very sensitive to radiation damage.

 You will get more specific suggestions what were the problems, such as ice thickness, radiation damage...


> 
> -- 
> There is a user in my facility that wants to plunge freeze liposomes for cryoem.  The problem is that the solvent composition is not aqueous.  It is 33% water, 11% ethanol, 14% glycerol, and 42%
> propylene glycol.    Has anyone ever tried something like this or is it even possible?  My first attempts didn't work.



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Masahide Kikkawa, M.D., Ph. D.
Professor
Department of Cell Biology
Graduate School of Medicine
University of Tokyo
http://structure.m.u-tokyo.ac.jp/
tel: +81-3-5841-3338
fax: +81-3-5841-3339
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