[3dem] Metal wire for shadowing experiments

[Christos Savva]

Hi

We do metal shadowing of RecA-coated DNA using an older Denton 502. I have been using tungsten wire (cat# 53110) purchased from Ladd Research. with the following specs: 

Single Strand Tungsten V-Filament - Single strand of
0.020" diameter tungsten wire.

This is the link:

http://www.laddresearch.com/Key_Products/Digital_High_Vacuum_Evaporator/VacEvap/Metal_Evaporation/Wires___Metals_for_Evaporation/wires___metals_for_evaporation.html

Hope this helps

Christos

>>> Anindito Sen <emailanindito at yahoo.co.in> 01/19/10 7:13 AM >>>
Dear all
I am looking for the correct metal (wire) for shadowing experiments to determine the handedness of my samples (biopolymers). The grain size   for needs to be fairly small. I will be using TMV as a standard for calibration. 
Please share your experience with such experiments and the source to obtain/purchase the metal.
Best Wishes
Andy



Dr. Anindito Sen (Ph.D)