[3dem] simulated micrograph for ctf determination?
Marin van Heel
m.vanheel at imperial.ac.uk
Fri Sep 11 03:21:34 PDT 2009
Dear Jinghua and Alex,
The IMAGIC command MODEL-MICROGRAPH will generate (stacks of)
micrographs with (for example) randomly distributed single particle
images. These single particle images are typically generated by
projecting a (set of) 3D PDB structure(s) in random orientations (using
the IMAGIC command THREED-MODEL-CREATION). MODEL-MICROGRAPH was created
to generate test data for automatic particle picking programs such as
our parallel PICK_M_ALL program. This latter program is aimed at picking
particles from huge sets of micrographs (astigmatic or non-astigmatic
data collection of large data sets on film or CCD). Once the noise-free
"micrographs" are created with MODEL-MICROGRAPH one would typically
first impose a CTF and then add realistic levels of noise depending on
the type of model data required.
Hope this helps,
Marin
===========================
jinghua tang wrote:
> To test various programs for determine the CTF, or simply the defocus,
> does anyone have
> a way to generate a simulated micrograph in which different CTF was
> applied?
>
> Thank you in advance for your kind helps!
>
> Jinghua
--
================================================================
Marin van Heel
Professor of Structural Biology
Division of Molecular Biosciences
Faculty of Natural Sciences
Imperial College London
Biochemistry Building
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