[3dem] Carbon evaporator

fang fang.zhao at helsinki.fi
Mon Oct 20 01:50:19 PDT 2008

Dear Yen-Ju:

If you always use pre-coated grids, the only occasion you need your coater
is when you need negative staining of the particle by sandwich method.


Fang Zhao, M.D. Ph.D.
Advanced Microscopy Unit
Department of Pathology
Haartman Institute
Haartmaninkatu 3, PL 21
00014 University of Helsinki

Tel. +358 9 1912 6261
E-mail: fang.zhao at helsinki.fi
Web: http://www.hi.helsinki.fi/amu/

-----Original Message-----
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of Yen-Ju Chen
Sent: Monday, October 20, 2008 6:35 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Carbon evaporator

Dear Colleagues

   We are considering to get a carbon evaporator here.
   The available model would be EMITECH K950X.
   I am wondering whether it is necessary to make our own carbon film
   while carbon-coated copper grids are available on the market.
   Is it worth to buy a carbon evaporator if we are only focusing on  
single-particle reconstruction
   and tomography with cryo-EM or with negative stain.

   We will appreciate your comments on this issue.


Yen-Ju Chen
Assistant Scientist
Life Science Group, Scientific Research Division
National Synchrotron Radiation Research Center

3dem mailing list
3dem at ncmir.ucsd.edu

More information about the 3dem mailing list