[3dem] Carbon evaporator

[fang]

Dear Yen-Ju:

If you always use pre-coated grids, the only occasion you need your coater
is when you need negative staining of the particle by sandwich method.

Regards,


Fang
----------------------------------------------------------------
Fang Zhao, M.D. Ph.D.
Advanced Microscopy Unit
Department of Pathology
Haartman Institute
Haartmaninkatu 3, PL 21
00014 University of Helsinki

 
Tel. +358 9 1912 6261
E-mail: fang.zhao at helsinki.fi
Web: http://www.hi.helsinki.fi/amu/

-----Original Message-----
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of Yen-Ju Chen
Sent: Monday, October 20, 2008 6:35 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Carbon evaporator

Dear Colleagues

   We are considering to get a carbon evaporator here.
   The available model would be EMITECH K950X.
   I am wondering whether it is necessary to make our own carbon film
   while carbon-coated copper grids are available on the market.
   Is it worth to buy a carbon evaporator if we are only focusing on  
single-particle reconstruction
   and tomography with cryo-EM or with negative stain.

   We will appreciate your comments on this issue.

   Regards

--
Yen-Ju Chen
Assistant Scientist
Life Science Group, Scientific Research Division
National Synchrotron Radiation Research Center

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