[3dem] EM sample in high salt

[Hong-Wei Wang]

Peijun,

You may want to consider using the cryo-negative staining protocol developed by Sacha De Carlo et al. on your specimen. We noticed that the saturated ammonium molybdate seems to mimic the high salt condition pretty well (particles that dislike high salt dissociate in the stain solution).

Hongwei
----------------------------
Hongwei Wang, Ph D
742 Stanley Hall
Berkeley, CA 94720
Tel: 510-666-3335
hwwang at lbl.gov
  ----- Original Message ----- 
  From: Peijun Zhang 
  To: 3dem at ncmir.ucsd.edu 
  Sent: Monday, July 07, 2008 3:15 PM
  Subject: [3dem] EM sample in high salt


  Hello all,

   

  I have an EM specimen which only stabilizes in high salt buffer (1M NaCl).  I would like to carry out cryoEM imaging at lower salt conditions.  Does anyone have a good protocol to reduce salt to 100mM or less without compromising the specimen?  

   

  Thanks so much,

   

  Best,

   

  Peijun

   



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