[3dem] EM sample in high salt

[Peijun Zhang]

Hello all,

 

I have an EM specimen which only stabilizes in high salt buffer (1M NaCl).
I would like to carry out cryoEM imaging at lower salt conditions.  Does
anyone have a good protocol to reduce salt to 100mM or less without
compromising the specimen?  

 

Thanks so much,

 

Best,

 

Peijun

 

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