[3dem] EM sample in high salt

[Florian Hauer]

Nat Methods. 2008 Jan;5(1):53-5. Epub 2007 Dec 23.


On Tue, 08 Jul 2008 00:15:44 +0200, Peijun Zhang <pez7+ at pitt.edu> wrote:

> Hello all,
>
>
> I have an EM specimen which only stabilizes in high salt buffer (1M  
> NaCl).
> I would like to carry out cryoEM imaging at lower salt conditions.  Does
> anyone have a good protocol to reduce salt to 100mM or less without
> compromising the specimen?
>
>
> Thanks so much,
>
>
> Best,
>
>
> Peijun
>
>



-- 
Florian Hauer

3D Cryo Electron Microscopy Group
Abt. 10710
Max-Planck Institute for biophysical Chemistry
Am Faßberg 11
37077 Göttingen

Phone: +49 551 201 1302
Fax:   +49 551 201 1197