[3dem] Airlock prepumping time

[Bill Tivol]

On Feb 22, 2008, at 10:32 AM, Eduardo Sanz-Garcia wrote:

> Our microscope allows to set the prepumping time for the airlock  
> when it is used in the supervisor mode.
> It was set to 90 sec and I modified to 30 sec. This makes the  
> inserting of the cryoholder faster and the temperature drops little.
> Could this short airlock prepumping time cause collateral problems  
> in the microscope?


Dear Eduardo,
	As long as the column vacuum remains satisfactory during insertion  
of the cryoholder, the pumping time is sufficient.  We use 50 sec on  
our T12.
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu

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