[3dem] Airlock prepumping time
bullitt at medusa.bu.edu
Fri Feb 22 10:43:55 PST 2008
We have always used 30 seconds with no problem. I think either way is
Esther Bullitt, Ph.D.
Dept. of Physiology & Biophysics, Room W302
Boston University School of Medicine
715 Albany Street (mail only; couriers
use 700 Albany Street)
Boston, MA 02118-2526
Email: bullitt at bu.edu
On Fri, 22 Feb 2008, Terje Dokland wrote:
> Hi Eduardo,
> I typically increase the pumping time to 120 sec when I am doing
> cryo. Although the temperature goes up initially, after about 1 min
> or so it drops again, and the vacuum is much better once the holder
> is inserted into the column.
> On Feb 22, 2008, at 12:32 PM, Eduardo Sanz-Garcia wrote:
> > Our microscope allows to set the prepumping time for the airlock
> > when it is used in the supervisor mode.
> > It was set to 90 sec and I modified to 30 sec. This makes the
> > inserting of the cryoholder faster and the temperature drops little.
> > Could this short airlock prepumping time cause collateral problems
> > in the microscope?
> > Thanks.
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