[3dem] Airlock prepumping time

Terje Dokland dokland at uab.edu
Fri Feb 22 10:36:47 PST 2008


Hi Eduardo,
I typically increase the pumping time to 120 sec when I am doing  
cryo. Although the temperature goes up initially, after about 1 min  
or so it drops again, and the vacuum is much better once the holder  
is inserted into the column.
Terje

On Feb 22, 2008, at 12:32 PM, Eduardo Sanz-Garcia wrote:

> Our microscope allows to set the prepumping time for the airlock  
> when it is used in the supervisor mode.
> It was set to 90 sec and I modified to 30 sec. This makes the  
> inserting of the cryoholder faster and the temperature drops little.
> Could this short airlock prepumping time cause collateral problems  
> in the microscope?
> Thanks.
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