[3dem] Zeiss SCAI/ ZI Photoscan discontinued

[Terje Dokland]

Like many others in the field, I also have the Nikon 9000ED and have  
been very pleased with it.

I am a little uneasy about my own home-made modification to the  
holder, though. (Done by shaving off the plastic ridge on both sides)  
Do you have a source for a more "professionally" modified holder? Do  
you use a glass holder? There is a commercially available glass  
holder but you'd have to cut the negative in half.

Thanks,
Terje


On Nov 15, 2007, at 1:45 PM, Steve Ludtke wrote:

> Hmm, I think I might have been called on this before... I think I  
> may be
> remembering what we paid for the 8000s just before the 9000s came  
> out. Indeed,
> the current price is closer to $2k. Nonetheless it is considerably  
> less than
> $130k...  Sorry for the confusion.
>
> ---------------------------------------------------------------------- 
> ------
> Steven Ludtke, PhD              |        Baylor College of Medicine
> sludtke at bcm.tmc.edu             |     Associate Professor & Co- 
> Director
> stevel at alumni.caltech.edu       | National Center For  
> Macromolecular Imaging
> V: (713)798-9020                |    Dept of Biochemistry and Mol.  
> Biol.
> F: (713)798-1625                |
>                                 |             Those who Do, Are
> http://ncmi.bcm.edu/~stevel     |         The converse also applies
>
> On Thu, 15 Nov 2007, Sacha De Carlo wrote:
>
>> Where can you buy a Nikon ED9000 for only 1k???
>> We have one but the lowest price I found was about $1800 [scanner  
>> only].
>>
>> Sacha
>> Boulder, CO
>>
>>
>> Steve Ludtke wrote:
>>
>>  We turned our Zeiss off about 3 years ago and haven't used it  
>> since. We now use
>> a set (they only cost ~$1k each) of Nikon 9000's. With a properly  
>> machined
>> holder (the ones they ship require some minor modifications), we  
>> found that they
>> outperformed our Zeiss in most respects. There is also a  
>> publication by Typke
>> describing some technical evaluations of these scanners.  Whenever  
>> a new
>> consumer grade scanner with apparent technical specifications that  
>> would make it
>> useful to cryoEM comes out we evaluate it, and so far none of the  
>> other
>> available 'consumer' grade scanners has performed as well as the  
>> Nikon (even
>> though on paper some look better).
>>
>>
>> --------------------------------------------------------------------- 
>> -------
>> Steven Ludtke, PhD              |        Baylor College of Medicine
>> sludtke at bcm.tmc.edu             |     Associate Professor & Co- 
>> Director
>> stevel at alumni.caltech.edu       | National Center For  
>> Macromolecular Imaging
>> V: (713)798-9020                |    Dept of Biochemistry and Mol.  
>> Biol.
>> F: (713)798-1625                |
>>                                 |             Those who Do, Are
>> http://ncmi.bcm.edu/~stevel     |         The converse also applies
>>
>> On Thu, 15 Nov 2007, Bob Grassucci wrote:
>>
>>
>>
>>  Hi All,
>> I was just wanted to inform everyone what was told to me yesterday  
>> by a
>> technician from Intergraphics.   Zeiss/Intergraphics has stopped
>> producing and selling the high resolution film scanner that many  
>> of us
>> are still using to digitize our EM film (SCAI , Photoscan 2000).   
>> They
>> installed their final one last week.  If you have one and are still
>> using it take care of it.  I am sure that as time goes on that  
>> support
>> for this discontinued device will disappear in the near future as  
>> well.
>> Many people are switching to CCD data collection but there are still
>> some who believe in the efficiency and resolving power of EM film.
>> Since there are many young investigators setting up labs for
>> macromolecular imaging it may be an appropriate time to survey the  
>> 3DEM
>> community as to the alternatives for digitization.  Thanks.
>>     Regards,
>>     Bob
>>
>> --
>> ********************************
>>
>> -Robert Grassucci-
>> Howard Hughes Medical Institute
>> Wadsworth Center Empire State Plaza
>> Albany, NY 12201-0509
>> bobg at wadsworth.org
>> Phone: (518)474-5821
>> Fax: (518)486-2191
>>
>>
>>
>>
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