[3DEM] 626 cryoholder pin

[Bill Tivol]

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On May 1, 2006, at 11:53 PM, Sharon G. Wolf wrote:

> We had problems with the pin breaking as well.
> Have you noticed if the pin is tilted in a specific direction?
>  Turns out that ours was being banged and
> bent when being pulled out from the pumping station. The pumping
> station has a little "ledge" which
> can catch on the pin and bend it. Our in-house technician adapted it so
> that this no longer happens.
>
Hi Sharon,
	Every time I have checked the pin I have seen it the right length and 
oriented perpendicular to the axis of the holder, and I have instructed 
all the users to check these two properties also and report any 
variation to me.  The two users who were on the scope when the pin 
broke are very trustworthy, so I am sure that the pin looked normal 
just before it broke.  Your idea about removing the ledge in the 
pumping station is a good one, and I'll have our machinist remove it on 
ours.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu

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