TEM: sucrose in cryo-specimens

[D. Prabha Dias, PhD]

I've been able to freeze samples with >5% sucrose by washing the 
sample once on the grid.  I would think 1% sucrose would wash out 
very easily, if you need to do it at all. As for image quality, I can 
only tell you that they are qualitatively comparable to samples 
without sucrose.  Does anyone have any data on this?

-Prabha

At 15:49 +0100 1/29/03, Philip Koeck wrote:
>Me again,
>
>would 1% sucrose be a problem in frozen hydrated specimens (single
>particles)?
>If so is it necessary to dyalize it out or is it sufficient to wash it of
>the grid before
>plunge-freezing?
>
>Thanks in advance,
>
>Philip
>
>Philip Koeck
>Svdertvrns Hvgskola and
>Karolinska Institutet
>Dept. of Bioscience at Novum
>S-14157 Huddinge
>Sweden
>phone: +46-8-6089186
>fax: +46-8-6089290

-- 
D. Prabha Dias, Ph.D.
Division of Biology, California Institute of Technology
M/C 114-96, BIO19
1200 East California Blvd.
Pasadena, CA 91125

phone: (626)395-8829
fax: (626)395-5730
email: prabha at caltech.edu

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